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- domestic rabbit monoclonal (JE64-63)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of eIF1A on different lysates with Rabbit anti-eIF1A antibody (HA721099) at 1/500 dilution. Lane 1: Jurkat cell lysate Lane 2: HepG2 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: PC-12 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 16 kDa Observed band size: 16 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721099) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-eIF1A antibody (HA721099) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721099) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier - mouse monoclonal (1B4)
reactivity: human, mouse
application: western blot, ELISA, immunocytochemistry, immunoprecipitation
gene information - mouse Eif1ax
- synonym:1500010B24Rik; AI426898; Eif1ay; eIF-4C
- description:eukaryotic translation initiation factor 1A, X-linked
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- we limit the listing of re-branded antibodies to provide our visitors with accurate information and the best experience
- we manually curate antibody information from the literature to obtain a comprehensive set of well-validated antibodies.
- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
product type
- Eif1ax antibody
- Eif1ax gene knockdown
- Eif1ax other
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