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- domestic rabbit polyclonal (S289-16)
reactivity: human, mouse
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section
citations: 5 - domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunoprecipitation, immunohistochemistry - paraffin section - domestic rabbit monoclonal (JE58-01)
reactivity: human, mouse
application: western blot, flow cytometry
Western blot analysis of CDT2 on different lysates with Rabbit anti-CDT2 antibody (HA721871) at 1/2,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: 293T cell lysate (20 µg/Lane) Lane 3: K-562 cell lysate (20 µg/Lane) Lane 4: Jurkat cell lysate (20 µg/Lane) Lane 5: Mouse spleen tissue lysate (40 µg/Lane) Predicted band size: 79 kDa Observed band size: 79 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721871) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Flow cytometric analysis of HeLa cells labeling CDT2. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721871, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 385.00 USD
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gene information - mouse Dtl
- synonym:2810047L02Rik; 5730564G15Rik; L2dtl; Ramp
- description:denticleless E3 ubiquitin protein ligase
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- Dtl antibody
- Dtl gene knockdown
- Dtl cDNA
- Dtl other
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