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- domestic rabbit polyclonal
reactivity: human, mouse
application: immunoprecipitation, immunohistochemistry - frozen section - mouse monoclonal (1-6)
reactivity: human, mouse
application: western blot
Western blot analysis on F9 cell lysates using anti-DPY30 Mouse mAb.
ICC staining DPY30 in F9 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue).
ICC staining DPY30 in NCCIT cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue).
quantity: 100μl
price: 360.00 USD
to the supplier - domestic rabbit polyclonal
reactivity: human, mouse
application: immunohistochemistry - paraffin section 
Immunocytochemistry analysis of NCCIT cells labeling DPY30 with Rabbit anti-DPY30 antibody (R1312-4) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-DPY30 antibody (R1312-4) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Immunocytochemistry analysis of F9 cells labeling DPY30 with Rabbit anti-DPY30 antibody (R1312-4) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-DPY30 antibody (R1312-4) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
ICC staining DPY30 in D3 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue).
quantity: 100μl
price: 330 USD
to the supplier
gene information - mouse Dpy30
- synonym:2810410M20Rik; C87842
- description:dpy-30, histone methyltransferase complex regulatory subunit
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- Dpy30 antibody
- Dpy30 gene knockdown
- Dpy30 cDNA
- Dpy30 other
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