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- domestic goat polyclonal
reactivity: mouse
application: western blot - domestic rabbit monoclonal (JE52-50)
reactivity: human, mouse
application: western blot, flow cytometry

Western blot analysis of DBF4 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7110-31, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: 293T cell lysate Lane 2: Hela cell lysate Lane 3: SW480 cell lysate Lane 4: Jurkat cell lysate
Flow cytometric analysis of DBF4 was done on F9 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7110-31, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 385.00 USD
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gene information - mouse Dbf4
- synonym:AA545217; Ask
- description:DBF4 zinc finger
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- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
product type
- Dbf4 antibody
- Dbf4 gene knockdown
- Dbf4 cDNA
- Dbf4 other
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