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- domestic rabbit polyclonal
reactivity: human, mouse, rat, chicken, zebrafish
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section
citations: 1 - domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of DACT1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500500, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse testis tissue lysate Lane 2: Mouse liver tissue lysate
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-DACT1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500500, 1/600) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 330 USD
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gene information - mouse Dact1
- synonym:4921528D17Rik; AI115603; DAPPER; DAPPER1; FRODO; Frd1; Frodo1; MDpr1; MTNG3; THYEX3
- description:dishevelled-binding antagonist of beta-catenin 1
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- we limit the listing of re-branded antibodies to provide our visitors with accurate information and the best experience
- we manually curate antibody information from the literature to obtain a comprehensive set of well-validated antibodies.
- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
product type
- Dact1 antibody
- Dact1 gene knockdown
- Dact1 cDNA
- Dact1 other
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