Your Filters
product
- mouse monoclonal (D-10)
reactivity: human, mouse
application: western blot, ELISA, immunocytochemistry, immunoprecipitation - mouse monoclonal (D-10)
reactivity: human, mouse
conjugate: agarose
application: immunoprecipitation - mouse monoclonal (D-10)
reactivity: human, mouse
conjugate: HRP
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section - domestic rabbit monoclonal (PSH03-88)
reactivity: human, mouse, rat
application: western blot
Western blot analysis of CRLF3 on different lysates with Rabbit anti-CRLF3 antibody (HA722054) at 1/2,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: Raji cell lysate (20 µg/Lane) Lane 3: Ramos cell lysate (20 µg/Lane) Lane 4: K-562 cell lysate (20 µg/Lane) Lane 5: Jurkat cell lysate (20 µg/Lane) Lane 6: 293T cell lysate (20 µg/Lane) Lane 7: A549 cell lysate (20 µg/Lane) Lane 8: RAW264.7 cell lysate (20 µg/Lane) Lane 9: PC-12 cell lysate (20 µg/Lane) Lane 10: Mouse thymus tissue lysate (40 µg/Lane) Lane 11: Mouse spleen tissue lysate (40 µg/Lane) Lane 12: Rat thymus tissue lysate (40 µg/Lane) Lane 13: Rat testis tissue lysate (40 µg/Lane) Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722054) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of RAW264.7 cells labeling CRLF3 with Rabbit anti-CRLF3 antibody (HA722054) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CRLF3 antibody (HA722054) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 385.00 USD
to the supplier - domestic rabbit monoclonal (PSH03-88)
reactivity: human, mouse, rat
application: western blot 
Western blot analysis of CRLF3 on different lysates with Rabbit anti-CRLF3 antibody (HA750913) at 1/2,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: Raji cell lysate (20 µg/Lane) Lane 3: Ramos cell lysate (20 µg/Lane) Lane 4: K-562 cell lysate (20 µg/Lane) Lane 5: Jurkat cell lysate (20 µg/Lane) Lane 6: 293T cell lysate (20 µg/Lane) Lane 7: A549 cell lysate (20 µg/Lane) Lane 8: RAW264.7 cell lysate (20 µg/Lane) Lane 9: PC-12 cell lysate (20 µg/Lane) Lane 10: Mouse thymus tissue lysate (40 µg/Lane) Lane 11: Mouse spleen tissue lysate (40 µg/Lane) Lane 12: Rat thymus tissue lysate (40 µg/Lane) Lane 13: Rat testis tissue lysate (40 µg/Lane) Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750913) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of RAW264.7 cells labeling CRLF3 with Rabbit anti-CRLF3 antibody (HA750913) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CRLF3 antibody (HA750913) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 649.00 USD
to the supplier
gene information - mouse Crlf3
- synonym:BB164954; Creme9; Cytor4
- description:cytokine receptor-like factor 3
do you know?
- we limit the listing of re-branded antibodies to provide our visitors with accurate information and the best experience
- we manually curate antibody information from the literature to obtain a comprehensive set of well-validated antibodies.
- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
product type
- Crlf3 antibody
- Crlf3 gene knockdown
- Crlf3 cDNA
- Crlf3 other
linkout
questions and comments