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- domestic rabbit polyclonal
reactivity: human, mouse
application: western blot
Western blot analysis of CPN1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500462, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: Raji cell lysate Lane 3: Mouse pancreas tissue lysate Predicted band size: 52 kDa Observed band size: 55 kDa
ICC staining of CPN1 in LOVO cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500462, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
quantity: 100μl
price: 330 USD
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gene information - mouse Cpn1
- synonym:0610011F20Rik; CPN
- description:carboxypeptidase N, polypeptide 1
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product type
- Cpn1 antibody
- Cpn1 gene knockdown
- Cpn1 cDNA
- Cpn1 other
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