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- domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, immunohistochemistry - free floating section
citations: 30
Published customer image: . Rabbit anti Mouse collagen 4 antibody ( 2150-1470 ) used for detectiuon of collagen 4 in rat retina by immunofluorescence on cryostat sections. Image caption: . Retinal gliosis and subretinal neovascularization in RCS rats. Double label immunostaining was performed using antibodies against vimentin (red) and collagen type IV (green) on frozen sections. (A) Non-dystrophic rat at 21 weeks (w) of age showed a normal distribution of retinal vessels confined to the inner retina and absence of glial cells in the subretinal space. (B, C) RCS rats at 6 w (B) and 9 w (C) of age showed activation of Muller cells but without subretinal neovascularization. Muller cell invasion into the subretinal space was more obvious at 9 w compared with 6 w of age (arrows in C). (D) Subretinal neovascularization (arrows) in RCS rats at 13 w of age. GCL = ganglion cell layer, INL = inner nuclear layer, OPL = outer plexiform layer, ONL = outer nuclear layer, SP = subretinal space, CC = choroidal capillaries. Scale bars: 50 um. From: Shen W, Chung SH, Irhimeh MR, Li S, Lee S-R, et al. (2014). Systemic Administration of Erythropoietin Inhibits Retinopathy in RCS Rats. PLoS ONE 9(8): e104759 .
quantity: 0.1 ml
price: 542 USD
to the supplier - domestic rabbit monoclonal (JB42-04)
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section 
Western blot analysis of KDEL on different lysates with Rabbit anti-KDEL antibody (ET7107-86) at 1/1,000 dilution. Lane 1: A549 cell lysate (20 µg/Lane) Lane 2: HepG2 cell lysate (20 µg/Lane) Lane 3: AGS cell lysate (20 µg/Lane) Lane 4: NIH/3T3 cell lysate (20 µg/Lane) Lane 5: Rat placenta tissue lysate (20 µg/Lane) Predicted band size: 25 kDa Observed band size: 50-100 kDa Exposure time: Lane 1-4: 1 minute; Lane 5: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-86) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of KDEL on different lysates with Rabbit anti-KDEL antibody (ET7107-86) at 1/1,000 dilution. Lane 1: RAW264.7 cell lysate (20 µg/Lane) Lane 2: C6 cell lysate (20 µg/Lane) Lane 3: Mouse placenta (E16.5) tissue lysate (20 µg/Lane) Predicted band size: 25 kDa Observed band size: 50-100 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-86) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of KDEL on different lysates with Rabbit anti-KDEL antibody (ET7107-86) at 1/1,000 dilution. Lane 1: Mouse lung tissue lysate (20 µg/Lane) Lane 2: Mouse colon tissue lysate (20 µg/Lane) Lane 3: Rat lung tissue lysate (20 µg/Lane) Lane 4: Rat colon tissue lysate (20 µg/Lane) Predicted band size: 25 kDa Observed band size: 50-100 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-86) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier - domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, ELISA, immunohistochemistry - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA, immunocytochemistry
gene information - mouse Col4a3
- synonym:[a]3(IV); alpha3(IV)
- description:collagen, type IV, alpha 3
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product type
- Col4a3 antibody
- Col4a3 gene knockdown
- Col4a3 cDNA
- Col4a3 other
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