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- domestic rabbit monoclonal
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, immunohistochemistry - paraffin section - domestic rabbit monoclonal
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, immunohistochemistry - paraffin section - domestic rabbit monoclonal (SY21-01)
reactivity: human, mouse, rat
application: western blot, flow cytometry
Western blot analysis of CDC7 Kinase on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1607-1, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Jurkat cell lysate Lane 2: Hela cell lysate
Flow cytometric analysis of CDC7 Kinase was done on A431 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1607-1, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 385.00 USD
to the supplier - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot
gene information - mouse Cdc7
- synonym:AI597260; Cdc7l1; muCdc7
- description:cell division cycle 7 (S. cerevisiae)
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product type
- Cdc7 antibody
- Cdc7 gene knockdown
- Cdc7 cDNA
- Cdc7 other
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