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- domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, flow cytometry
Western blot analysis of CCPG1 on different lysates with Rabbit anti-CCPG1 antibody (HA500520) at 1/1,000 dilution. Lane 1: HCT116 cell lysate Lane 2: A549 cell lysate Lane 3: Hela cell lysate Lane 4: THP-1 cell lysate Lane 5: SW480 cell lysate Lane 6: HEK-293 cell lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 87 kDa Observed band size: 105 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500520) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Western blot analysis of CCPG1 on different lysates with Rabbit anti-CCPG1 antibody (HA500520) at 1/1,000 dilution. Lane 1: NIH/3T3 cell lysate Lane 2: C2C12 cell lysate Lane 3: MEF cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 87 kDa Observed band size: 105 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500520) at 1/1,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Flow cytometric analysis of SW480 cells labeling CCPG1. Cells were fixed and permeabilized. Then stained with the primary antibody (HA500520, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 330 USD
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gene information - mouse Ccpg1
- synonym:1700030B06Rik; 1810073J13Rik; 9430028F23Rik; AI426686; AI875170; CPR8; D9Ertd392e
- description:cell cycle progression 1
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product type
- Ccpg1 antibody
- Ccpg1 gene knockdown
- Ccpg1 cDNA
- Ccpg1 other
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