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- domestic rabbit polyclonal
reactivity: mouse
application: western blot, immunohistochemistry - paraffin section - domestic sheep polyclonal
reactivity: mouse, rat
application: western blot

Detection of Rat BMP-15/GDF-9B antibody by Western Blot.
quantity: 100 ug (also 25 ug)
price: 469 USD
to the supplier - rat monoclonal (582703)
reactivity: mouse
application: immunohistochemistry

BMP-15/GDF-9B antibody in Mouse Ovary by Immunohistochemistry (IHC-Fr).
quantity: 100 ug (also 25 ug)
price: 419 USD
to the supplier - domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, ELISA - domestic rabbit monoclonal (JE51-47)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of BMP15 on different lysates with Rabbit anti-BMP15 antibody (ET7110-03) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: SK-OV-3 cell lysate (20 µg/Lane) Lane 3: Mouse testis tissue lysate (20 µg/Lane) Lane 4: Rat testis tissue lysate (20 µg/Lane) Predicted band size: 45 kDa Observed band size: 50 kDa Exposure time: 5 minutes 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-03) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue with Rabbit anti-BMP15 antibody (ET7110-03) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-03) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-BMP15 antibody (ET7110-03) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-03) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier
gene information - mouse Bmp15
- synonym:AU015375; AU018861; AU021453; Bmp-15; C86824; C87336; GDF-9B
- description:bone morphogenetic protein 15
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- we limit the listing of re-branded antibodies to provide our visitors with accurate information and the best experience
- we manually curate antibody information from the literature to obtain a comprehensive set of well-validated antibodies.
- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
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