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- domestic rabbit monoclonal (JE64-60)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of ATP5G1 on different lysates with Rabbit anti-ATP5G1 antibody (HA722921) at 1/5,000 dilution. Lane 1: HeLa cell lysate (10 µg/Lane) Lane 2: Human liver tissue lysate (20 µg/Lane) Lane 3: Mouse liver tissue lysate (20 µg/Lane) Lane 4: Mouse kidney tissue lysate (10 µg/Lane) Lane 5: Rat liver tissue lysate (10 µg/Lane) Predicted band size: 14 kDa Observed band size: 8 kDa Exposure time: 2 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722921) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded rat small intestine tissue with Rabbit anti-ATP5G1 antibody (HA722921) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722921) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier - domestic rabbit monoclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section - domestic rabbit monoclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot
gene information - mouse Atp5g1
- synonym:Atp5mc1
- description:ATP synthase, H+ transporting, mitochondrial F0 complex, subunit C1 (subunit 9)
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product type
- Atp5g1 antibody
- Atp5g1 gene knockdown
- Atp5g1 cDNA
- Atp5g1 other
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