Your Filters
product
- mouse monoclonal (G-3)
reactivity: human, mouse
application: western blot, ELISA, immunocytochemistry, immunoprecipitation - mouse monoclonal (G-3)
reactivity: human, mouse
conjugate: agarose
application: immunoprecipitation - mouse monoclonal (G-3)
reactivity: human, mouse
conjugate: HRP
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section - mouse monoclonal (11B3)
reactivity: human, mouse, rat
application: western blot, flow cytometry
Western blot analysis of Angiotensin II Type 1 Receptor on different lysates with Mouse anti-Angiotensin II Type 1 Receptor antibody (HA600029) at 1/500 dilution. Lane 1: PC-12 cell lysate Lane 2: A549 cell lysate Lane 3: HepG2 cell lysate Lane 4: HeLa cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 41 kDa Observed band size: 49 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600029) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/200,000 dilution was used for 1 hour at room temperature.
ICC staining of Angiotensin II Type 1 Receptor in PC-12 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA600029, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ICC staining of Angiotensin II Type 1 Receptor in 293T cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA600029, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
quantity: 100μl
price: 360.00 USD
to the supplier
gene information - mouse Agtr1b
- synonym:AT1B; AT2R1B; Agtr-1b; Angtr-1b
- description:angiotensin II receptor, type 1b
do you know?
- we limit the listing of re-branded antibodies to provide our visitors with accurate information and the best experience
- we manually curate antibody information from the literature to obtain a comprehensive set of well-validated antibodies.
- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
product type
- Agtr1b antibody
- Agtr1b gene knockdown
- Agtr1b cDNA
- Agtr1b other
species
linkout
questions and comments