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- domestic rabbit polyclonal
reactivity: human, mouse
application: western blot - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, flow cytometry
Western blot analysis of AFF4 on different lysates with Rabbit anti-AFF4 antibody (HA500590) at 1/20,000 dilution. Lane 1: MCF7 cell lysate Lane 2: HeLa cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: SP2/0 cell lysate Lane 5: Rat pancreas tissue lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 127 kDa Observed band size: 150 kDa Exposure time: Lane 1-4: 1 minute 12 seconds; Lane 5: 9 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500590) at 1/20,000 dilution was used in 5% BSA at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of HeLa cells labeling AFF4 with Rabbit anti-AFF4 antibody (HA500590) at 1/1,000 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-AFF4 antibody (HA500590) at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Flow cytometric analysis of HeLa cells labeling AFF4. Cells were fixed and permeabilized. Then stained with the primary antibody (HA500590, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 330 USD
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gene information - mouse Aff4
- synonym:AF5Q31; Alf4; Laf4l; MCEF
- description:AF4/FMR2 family, member 4
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- Aff4 antibody
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