Your Filters
product
- domestic rabbit monoclonal
reactivity: human, mouse
application: western blot, immunoprecipitation - domestic rabbit polyclonal
reactivity: human, mouse
application: western blot - domestic rabbit monoclonal
reactivity: human, mouse
application: western blot, immunoprecipitation - domestic rabbit monoclonal (PSH07-31)
reactivity: mouse
application: western blot
Western blot analysis of IRG1 on different lysates with Rabbit anti-IRG1 antibody (HA722819) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution. Lane 1: RAW264.7 cell lysate Lane 2: RAW264.7 treated with 0.1μg/mL LPS for 6 hours cell lysate Lane 3: J774A.1 cell lysate Lane 4: J774A.1 treated with 1μg/mL LPS for 24 hours cell lysate Lane 5: mBMMC cell lysate Lane 6: mBMMC treated with 0.1μg/mL LPS and 10ng/mL IFN-gamma for 24 hours cell lysate Lane 7: mBMSC cell lysate Lane 8: mBMSC treated with 40ng/mL MCSF for 7 days then add 100ng/mL LPS for 48 hours cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722819) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of RAW264.7 cells treated with 0.1μg/mL LPS for 6 hours labeling IRG1 with Rabbit anti-IRG1 antibody (HA722819) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-IRG1 antibody (HA722819) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 385.00 USD
to the supplier - domestic rabbit monoclonal (PSH07-31)
reactivity: mouse
application: western blot 
Western blot analysis of IRG1 on different lysates with Rabbit anti-IRG1 antibody (HA751152) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution. Lane 1: RAW264.7 cell lysate Lane 2: RAW264.7 treated with 0.1μg/mL LPS for 6 hours cell lysate Lane 3: J774A.1 cell lysate Lane 4: J774A.1 treated with 1μg/mL LPS for 24 hours cell lysate Lane 5: mBMMC cell lysate Lane 6: mBMMC treated with 0.1μg/mL LPS and 10ng/mL IFN-gamma for 24 hours cell lysate Lane 7: mBMSC cell lysate Lane 8: mBMSC treated with 40ng/mL MCSF for 7 days then add 100ng/mL LPS for 48 hours cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751152) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of RAW264.7 cells treated with 0.1μg/mL LPS for 6 hours labeling IRG1 with Rabbit anti-IRG1 antibody (HA751152) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-IRG1 antibody (HA751152) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 649.00 USD
to the supplier
gene information - mouse Acod1
- synonym:AI323667; CAD; Irg1
- description:aconitate decarboxylase 1
do you know?
- we limit the listing of re-branded antibodies to provide our visitors with accurate information and the best experience
- we manually curate antibody information from the literature to obtain a comprehensive set of well-validated antibodies.
- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
product type
species
linkout
questions and comments