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- rat monoclonal (7A1-144)
reactivity: human, mouse
application: western blot, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section
citations: 2 - rat monoclonal (7A1-144)
reactivity: human, mouse
application: western blot, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section

Western Blot: ABCA7 Antibody (7A1-144) [NB400-166] - Detection of ABCA7 using NB 400-166 at various dilutions in ABCA7 transfected HeLa cell lysates.
quantity: 0.1 ml (also 0.025 ml)
price: 349 USD
to the supplier - rat monoclonal (7A1-144)
reactivity: human, mouse
application: western blot, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section - domestic rabbit monoclonal (PSH18-11)
reactivity: mouse, rat
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of ABCA7 on different lysates with Rabbit anti-ABCA7 antibody (HA723974) at 1/5,000 dilution. Lane 1: Neuro-2a cell lysate (no heat) (20 µg/Lane) Lane 2: Mouse brain tissue lysate (no heat) (40 µg/Lane) Lane 3: Mouse cerebellum (P7) tissue lysate (40 µg/Lane) Notice: no heat means the lysate is not boiled. Predicted band size: 234 kDa Observed band size: 250 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723974) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded mouse thymus tissue with Rabbit anti-ABCA7 antibody (HA723974) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723974) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-ABCA7 antibody (HA723974) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723974) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier - rat monoclonal (7A1-144)
reactivity: mouse
application: western blot, immunocytochemistry, immunoprecipitation, flow cytometry
gene information - mouse Abca7
- synonym:ABCX; Abc51
- description:ATP-binding cassette, sub-family A (ABC1), member 7
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- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
product type
- Abca7 antibody
- Abca7 gene knockdown
- Abca7 cDNA
- Abca7 other
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