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- mouse monoclonal (1A8)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, flow cytometry
Western blot analysis of USP29 on different lysates with Rabbit anti-USP29 antibody (ER1902-76) at 1/500 dilution. Lane 1: Rat testis tissue lysate Lane 2: Mouse testis tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 104 kDa Observed band size: 104 kDa Exposure time: 12 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1902-76) at 1/500 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of USP29 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-76, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: THP-1 cell lysate Lane 2: Rat testis tissue lysate
Flow cytometric analysis of USP29 was done on 293 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1902-76, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 330 USD
to the supplier - domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot
gene information - human ubiquitin specific processing protease
- synonym:HOM-TES-84/86
- description:ubiquitin specific peptidase 29
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product type
- ubiquitin specific processing protease antibody
- ubiquitin specific processing protease gene knockdown
- ubiquitin specific processing protease cDNA
- ubiquitin specific processing protease protein
- ubiquitin specific processing protease other
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