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- domestic rabbit monoclonal
reactivity: human
application: western blot - domestic rabbit monoclonal
reactivity: human
application: western blot - domestic rabbit monoclonal (JE35-00)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of Neuronatin on different lysates with Rabbit anti-Neuronatin antibody (HA722326) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: SK-Br-3 cell lysate (negative) Lane 3: E14.0 mouse brain tissue lysate Lane 4: Mouse embryo tissue lysate Lane 5: Rat embryo tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 9 kDa Observed band size: 12 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722326) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Neuronatin antibody (HA722326) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722326) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
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gene information - human neuronatin
- synonym:Peg5
- description:neuronatin
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- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
product type
- neuronatin antibody
- neuronatin gene knockdown
- neuronatin cDNA
- neuronatin other
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