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- domestic sheep polyclonal
reactivity: human, mouse
application: immunohistochemistry, immunocytochemistry
citations: 2
ATBF1/ZFHX3 antibody in MCF-7 Human Cell Line by Immunocytochemistry (ICC).
quantity: 100 ug (also 25 ug)
price: 469 USD
to the supplier - domestic rabbit polyclonal
reactivity: human
application: immunohistochemistry - mouse monoclonal (3B1)
reactivity: human, mouse
application: western blot, ELISA, immunocytochemistry, immunoprecipitation - domestic rabbit polyclonal
reactivity: human, rat
application: western blot, immunohistochemistry - paraffin section 
Western blot analysis of ATBF1 on ATBF1 recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500181) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: ATBF1 recombinant protein with primary antibody at 1:1,000 dilutions Lane 2: ATBF1 recombinant protein with primary antibody at 1:5,000 dilutions
Immunohistochemical analysis of paraffin-embedded rat esophagus tissue using anti-ATBF1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500181, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-ATBF1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500181, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 330 USD
to the supplier - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, flow cytometry 
Western blot analysis of ATBF1 on MCF-7 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-49, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Flow cytometric analysis of ATBF1 was done on HepG2 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER2001-49, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 330 USD
to the supplier
gene information - human ZFHX3
- synonym:ATBF1; ATBT; C16orf47; ZFH-3; ZNF927
- description:zinc finger homeobox 3
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- we limit the listing of re-branded antibodies to provide our visitors with accurate information and the best experience
- we manually curate antibody information from the literature to obtain a comprehensive set of well-validated antibodies.
- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
product type
- ZFHX3 antibody
- ZFHX3 gene knockdown
- ZFHX3 cDNA
- ZFHX3 protein
- ZFHX3 other
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