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- domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, enzyme immunoassay
Western Blot. Positive WB detected in: HepG2 whole cell lysate, 293 whole cell lysate, Jurkat whole cell lysate, A549 whole cell lysate. All lanes: ZDHHC14 antibody at 10.5ug/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 54, 52 KDa. Observed band size: 54 KDa.
IHC image of MBS7110323 diluted at 1:600 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of HepG2 cells with MBS7110323 at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
quantity: 0.05 mg
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to the supplier - domestic rabbit polyclonal
reactivity: human
application: western blot - domestic rabbit polyclonal
reactivity: human
application: western blot - domestic rabbit polyclonal
reactivity: human
application: immunocytochemistry
gene information - human ZDHHC14
- synonym:NEW1CP
- description:zinc finger DHHC-type palmitoyltransferase 14
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product type
- ZDHHC14 antibody
- ZDHHC14 gene knockdown
- ZDHHC14 cDNA
- ZDHHC14 other
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