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- mouse monoclonal (A6-A11-9)
reactivity: human
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of TMEM177 on recombinant protein using anti-TMEM177 antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-TMEM177 antibody (M1306-1) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1306-1) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC staining TMEM177 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
quantity: 100μl
price: 360.00 USD
to the supplier - domestic rabbit polyclonal
reactivity: human
application: western blot
gene information - human TMEM177
- description:transmembrane protein 177
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product type
- TMEM177 antibody
- TMEM177 gene knockdown
- TMEM177 cDNA
- TMEM177 protein
- TMEM177 other
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