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- domestic rabbit monoclonal (JE66-67)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section - domestic rabbit monoclonal (JE66-67)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of TIMM22 on different lysates with Rabbit anti-TIMM22 antibody (HA721373) at 1/1,000 dilution. Lane 1: K-562 cell lysate (15 µg/Lane) Lane 2: HepG2 cell lysate (15 µg/Lane) Lane 3: A549 cell lysate (15 µg/Lane) Lane 4: MCF7 cell lysate (15 µg/Lane) Lane 5: HEK-293 cell lysate (15 µg/Lane) Lane 6: HeLa cell lysate (15 µg/Lane) Lane 7: Jurkat cell lysate (15 µg/Lane) Lane 8: Mouse testis tissue lysate (30 µg/Lane) Predicted band size: 20 kDa Observed band size: 20 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721373) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-TIMM22 antibody (HA721373) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721373) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-TIMM22 antibody (HA721373) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721373) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier - domestic rabbit monoclonal
reactivity: human
application: western blot - domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry - paraffin section - domestic rabbit monoclonal
reactivity: human
application: western blot
gene information - human TIMM22
- synonym:TEX4; TIM22
- description:translocase of inner mitochondrial membrane 22
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- we limit the listing of re-branded antibodies to provide our visitors with accurate information and the best experience
- we manually curate antibody information from the literature to obtain a comprehensive set of well-validated antibodies.
- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
product type
- TIMM22 antibody
- TIMM22 gene knockdown
- TIMM22 cDNA
- TIMM22 other
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