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- domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA, enzyme immunoassay - domestic rabbit monoclonal
reactivity: human
application: western blot, immunocytochemistry, immunohistochemistry - paraffin section - domestic rabbit monoclonal
reactivity: human
application: western blot, immunocytochemistry, immunohistochemistry - paraffin section - domestic rabbit polyclonal
reactivity: human
application: western blot - domestic rabbit monoclonal (JE64-97)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of TAF3 on 293 cell lysates with Rabbit anti-TAF3 antibody (HA721107) at 1/1,000 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 104 kDa Observed band size: 140 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721107) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human thyroid carcinoma tissue with Rabbit anti-TAF3 antibody (HA721107) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721107) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-TAF3 antibody (HA721107) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721107) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
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gene information - human TAF3
- synonym:TAF140; TAFII-140; TAFII140
- description:TATA-box binding protein associated factor 3
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- we manually curate antibody information from the literature to obtain a comprehensive set of well-validated antibodies.
- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
product type
- TAF3 antibody
- TAF3 gene knockdown
- TAF3 cDNA
- TAF3 other
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