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- mouse monoclonal (5D11.G5)
reactivity: human, mouse
modification: phosphorylated
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section
citations: 3
Western blot of gamma irradiated (+ lanes) and mock-irradiated (- lanes) human GM536 lymphoblastoid cell lysate (left panel) and mouse NIH3T3 cell lysate (right panel). Rockland's Protein G Purified Mab anti-SMC1 pS957 detects a 160 kDa band corresponding to phosphorylated SMC1. The antibody does not react with non-phosphorylated SMC1 present in the human control lane. Non specific binding may occur in control lanes of lysates from mouse cell origins. The cell lysates were prepared in a RIPA buffer containing 200 mM NaCl, and 20 ?g protein was loaded per lane. A 4-12% Bis-Tris gradient gel (Invitrogen) was used for SDS-PAGE. The membrane was probed with the primary antibody at 10??g/ml for 1 h at 20°C followed by washes and reaction with a 1:1000 dilution of HRP conjugated Dnky-a-Mouse IgG [H&L] (code 610-703-124) for 30 min.
quantity: 100 µg
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to the supplier - domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry - paraffin section - mouse monoclonal (C2M)
reactivity: human
application: western blot, immunoprecipitation - domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry - domestic rabbit polyclonal
reactivity: human
application: immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
gene information - human SMC1B
- synonym:SMC1BETA; SMC1L2
- description:structural maintenance of chromosomes 1B
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- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
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