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- domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of Mitoferrin1 on mouse spleen tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500278, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Predicted band size: 37 kDa Observed band size: 37 kDa
Western blot analysis of Mitoferrin1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500278, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse brain tissue lysate Lane 2: THP-1 cell lysate Predicted band size: 37 kDa Observed band size: 37 kDa
ICC staining of Mitoferrin1 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500278, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
quantity: 100μl
price: 330 USD
to the supplier - domestic rabbit polyclonal
reactivity: human
application: western blot
gene information - human SLC25A37
- synonym:HT015; MFRN; MFRN1; MSC; MSCP; PRO1278; PRO1584; PRO2217
- description:solute carrier family 25 member 37
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product type
- SLC25A37 antibody
- SLC25A37 gene knockdown
- SLC25A37 cDNA
- SLC25A37 other
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