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- domestic rabbit monoclonal (JE64-36)
reactivity: human
application: western blot, flow cytometry, immunohistochemistry - paraffin section
Western blot analysis of RAVER2 on different lysates with Rabbit anti-RAVER2 antibody (HA721100) at 1/5,000 dilution. Lane 1: 293T cell lysate Lane 2: Jurkat cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 74 kDa Observed band size: 74 kDa Exposure time: 46 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721100) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human cervix carcinoma tissue with Rabbit anti-RAVER2 antibody (HA721100) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721100) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Flow cytometric analysis of Jurkat cells labeling RAVER2. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721100, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 385.00 USD
to the supplier - domestic rabbit polyclonal
reactivity: human
application: western blot - domestic rabbit monoclonal
reactivity: human
application: western blot, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section - domestic rabbit monoclonal
reactivity: human
application: western blot, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section
gene information - human RAVER2
- description:ribonucleoprotein, PTB binding 2
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product type
- RAVER2 antibody
- RAVER2 gene knockdown
- RAVER2 cDNA
- RAVER2 other
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