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- domestic goat polyclonal
reactivity: human
application: western blot - domestic rabbit polyclonal (RB48083)
reactivity: human
application: western blot - domestic rabbit polyclonal
reactivity: human
application: western blot - domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, flow cytometry
Western blot analysis of PHF11 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500478, 1/5,000) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Raji cell lysate Lane 2: Jurkat cell lysate
Western blot analysis of PHF11 on mouse testis tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500478, 1/1,000) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of HEK-293 cells labeling PHF11 with Rabbit anti-PHF11 antibody (HA500478) at 1/2,000 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PHF11 antibody (HA500478) at 1/2,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 330 USD
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gene information - human PHF11
- synonym:APY; BCAP; IGEL; IGER; IGHER; NY-REN-34; NYREN34
- description:PHD finger protein 11
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product type
- PHF11 antibody
- PHF11 gene knockdown
- PHF11 cDNA
- PHF11 protein
- PHF11 other
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