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- mouse monoclonal (257934)
reactivity: human, mouse
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - frozen section
citations: 4 - mouse monoclonal (257914)
reactivity: human, mouse
application: immunohistochemistry
Olig3 antibody in 13 dpc Mouse Embryo Tissue by Immunohistochemistry (IHC-Fr).
quantity: 100 ug (also 25 ug)
price: 389 USD
to the supplier - domestic rabbit monoclonal (JE56-40)
reactivity: human, mouse
application: western blot, immunohistochemistry - paraffin section 
Western blot analysis of Olig3 on human placenta tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA720016, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
ICC staining of Olig3 in F9 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA720016, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Olig3 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720016, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier - domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, flow cytometry, immunohistochemistry - paraffin section - domestic rabbit polyclonal
reactivity: human
application: western blot
gene information - human OLIG3
- synonym:Bhlhb7; bHLHe20
- description:oligodendrocyte transcription factor 3
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- we manually curate antibody information from the literature to obtain a comprehensive set of well-validated antibodies.
- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
product type
- OLIG3 antibody
- OLIG3 gene knockdown
- OLIG3 cDNA
- OLIG3 protein
- OLIG3 other
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