NSUN3
HUABIO
catalog: HA721755

domestic rabbit monoclonal (PSH01-97)
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of NSUN3 on different lysates with Rabbit anti-NSUN3 antibody (HA721755) at 1/1,000 dilution. Lane 1: HeLa cell lysate (40 µg/Lane) Lane 2: K-562 cell lysate (40 µg/Lane) Lane 3: HL-60 cell lysate (40 µg/Lane) Lane 4: 293T cell lysate (40 µg/Lane) Lane 5: Mouse thymus tissue lysate (40 µg/Lane) Lane 6: Mouse colon tissue lysate (40 µg/Lane) Lane 7: Mouse testis tissue lysate (40 µg/Lane) Predicted band size: 38 kDa Observed band size: 38 kDa Exposure time: 3 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721755) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of NSUN3 on different lysates with Rabbit anti-NSUN3 antibody (HA721755) at 1/1,000 dilution. Lane 1: RAW264.7 cell lysate (30 µg/Lane) Lane 2: C2C12 cell lysate (30 µg/Lane) Lane 3: Neuro-2a cell lysate (30 µg/Lane) Lane 4: PC-12 cell lysate (30 µg/Lane) Predicted band size: 38 kDa Observed band size: 38 kDa Exposure time: 1 minute 59 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721755) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-NSUN3 antibody (HA721755) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721755) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier

NSUN3
HUABIO
catalog: HA750771

domestic rabbit monoclonal (PSH01-97)
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of NSUN3 on different lysates with Rabbit anti-NSUN3 antibody (HA750771) at 1/1,000 dilution. Lane 1: HeLa cell lysate (40 µg/Lane) Lane 2: K-562 cell lysate (40 µg/Lane) Lane 3: HL-60 cell lysate (40 µg/Lane) Lane 4: 293T cell lysate (40 µg/Lane) Lane 5: Mouse thymus tissue lysate (40 µg/Lane) Lane 6: Mouse colon tissue lysate (40 µg/Lane) Lane 7: Mouse testis tissue lysate (40 µg/Lane) Predicted band size: 38 kDa Observed band size: 38 kDa Exposure time: 3 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750771) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of NSUN3 on different lysates with Rabbit anti-NSUN3 antibody (HA750771) at 1/1,000 dilution. Lane 1: RAW264.7 cell lysate (30 µg/Lane) Lane 2: C2C12 cell lysate (30 µg/Lane) Lane 3: Neuro-2a cell lysate (30 µg/Lane) Lane 4: PC-12 cell lysate (30 µg/Lane) Predicted band size: 38 kDa Observed band size: 38 kDa Exposure time: 1 minute 59 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750771) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-NSUN3 antibody (HA750771) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750771) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 649.00 USD
to the supplier

Anti-NSUN3 antibody
Abcam
catalog: ab272616

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry - paraffin section

quantity: 100µL
price:
to the supplier

NSUN3 Antibody (PSH01-97)
Novus Biologicals
catalog: NBP3-32665

domestic rabbit monoclonal (PSH01-97)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

NSUN3 Antibody (PSH01-97)
quantity: 100 ul
price: 449 USD
to the supplier