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- mouse monoclonal (N187/7 (Formerly sold as S187-7))
reactivity: human, mouse, rat
application: western blot, immunocytochemistry
citations: 5
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-NALCN Monoclonal Antibody, Clone S187-7 (SMC-417). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-NALCN Monoclonal Antibody (SMC-417) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) NALCN Antibody. (D) Composite.
quantity: 100 µg
price:
to the supplier - domestic rabbit polyclonal (NA)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry
citations: 4 
Western blot analysis of rat brain (lanes 1 and 4), mouse heart (lanes 2 and 5) and human SH-SYS5 brain neuroblastoma cell (lanes 3 and 6) lysates: - 1-3. Anti-NALCN/VGCNL1 (extracellular) Antibody (#ASC-022), (1:200).4-6. Anti-NALCN/VGCNL1 (extracellular) Antibody, preincubated with NALCN/VGCNL1 (extracellular) Blocking Peptide (#BLP-SC022).
Expression of NALCN in rat hypothalamus - Immunohistochemical staining of rat hypothalamus using Anti-NALCN/VGCNL1 (extracellular) Antibody (#ASC-022). A. NALCN (green) staining appears in the supra-optic nucleus (SON). B. DAPI is used as the counterstain (blue). White arrows point at the sharp border between the SON and optic tract (OT). C. Merged images of A and B.
Expression ofNALCN in rat PC12 cells - Cell surface detection ofNALCN in intact living rat pheochromocytoma PC12 cells. Extracellular staining of cells usingAnti-NALCN/VGCNL1 (extracellular) Antibody(#ASC-022) (1:50) followed by goat anti-rabbit-AlexaFluor-594secondary antibody. Merged image of extracellular staining with live image of the cells.
quantity:
price:
to the supplier - domestic rabbit monoclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section - mouse monoclonal (S187-7)
reactivity: human, mouse, rat
application: western blot, immunocytochemistry 
Western Blot: VGCNL1 Antibody (S187-7) [NBP2-22410] - Western Blot analysis of Rat Brain showing detection of ~200 kDa VGCNL1protein using Mouse Anti-VGCNL1Monoclonal Antibody, Clone S187-7 (NBP2-22410). Lane 1: Molecular Weight (MW) Ladder. Lane 3: Rat Brain Membrane. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-VGCNL1Monoclonal Antibody (NBP2-22410) at 1:1000 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~200 kDa.
quantity: 0.1 mg (also 0.025 mg)
price: 459 USD
to the supplier
gene information - human NALCN
- synonym:CLIFAHDD; CanIon; IHPRF; IHPRF1; INNFD; VGCNL1; bA430M15.1
- description:sodium leak channel, non-selective
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- we limit the listing of re-branded antibodies to provide our visitors with accurate information and the best experience
- we manually curate antibody information from the literature to obtain a comprehensive set of well-validated antibodies.
- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
Article
product type
- NALCN antibody
- NALCN gene knockdown
- NALCN cDNA
- NALCN other
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