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- domestic goat polyclonal
reactivity: human
application: western blot - domestic rabbit polyclonal
reactivity: human
application: western blot - domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, flow cytometry, immunohistochemistry - paraffin section
Western blot analysis of MTHFD2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-98, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HL-60 cell lysate Lane 2: 293T cell lysate
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-MTHFD2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-98, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-MTHFD2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-98, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 330 USD
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gene information - human MTHFD2L
- description:methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2 like
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product type
- MTHFD2L antibody
- MTHFD2L gene knockdown
- MTHFD2L cDNA
- MTHFD2L protein
- MTHFD2L other
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