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- domestic rabbit polyclonal (NA)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry
citations: 7
Western blot analysis of rat kidney (lanes 1 and 3) and liver (lanes 2 and 4) membranes: - 1,2. Anti-Kir5.1 Antibody (#APC-123), (1:200).3,4. Anti-Kir5.1 Antibody, preincubated with Kir5.1 Blocking Peptide (#BLP-PC123).
Expression of Kir5.1 in rat kidney - Immunohistochemical staining of paraffin embedded section of rat kidney using Anti-Kir5.1 Antibody (#APC-123) (1:100). Kir5.1 (brown staining) is expressed in both proximal tubules (PT) and distal tubules (DT) in the renal cortex. Note that collecting ducts (CD) are less stained while glomeruli (G) are negative. Hematoxilin is used as the counterstain.
Western blot analysis of rat kidney (lanes 1 and 3) and liver (lanes 2 and 4) membranes: - 12.Anti-Kir5.1Antibody (#APC-123) (1:200).34.Anti-Kir5.1 Antibody preincubated with the control peptide antigen.
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to the supplier - domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section 
Western blot analysis of Kir5.1 on HEK-293 cell lysates with Rabbit anti-Kir5.1 antibody (ER1901-24) at 1/2,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 48 kDa Observed band size: 48 kDa Exposure time: 35 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1901-24) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Kir5.1 on mouse kidney tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-24, 1/2000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Kir5.1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-24, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 330 USD
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gene information - human KCNJ16
- synonym:BIR9; KIR5.1
- description:potassium inwardly rectifying channel subfamily J member 16
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