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- mouse monoclonal (S124B-38)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry
Western Blot of mouse anti-Kir2.2 antibody. Lane 1: Human Cell line mix. Primary antibody: Kir2.2 antibody at 1:1000 for overnight at 4°C. Secondary antibody: Goat anti-mouse IgG HRP secondary antibody at 1:10,000 for 45 min at RT. Block: 5% Blotto overnight 4°C. Predicted/Observed size: 48.4 kDa/48 kDa for Kir2.2 total. Other band(s): none.
quantity: 100 µg
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to the supplier - domestic rabbit polyclonal
reactivity: human
application: immunohistochemistry - paraffin section - domestic rabbit polyclonal (NA)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry 
Western blot analysis of rat kidney (lanes 1 and 3) and brain (lanes 2 and 4) membranes: - 1,2. Anti-Kir2.2 (KCNJ12) Antibody (#APC-042), (1:200). 3,4. Anti-Kir2.2 (KCNJ12) Antibody, preincubated with Kir2.2/KCNJ12 Blocking Peptide (#BLP-PC042).
Expression of Kir2.2 in rat cerebellum. - Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Kir2.2 (KCNJ12) Antibody (#APC-042), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. Kir2.2 immunoreactivity (green) appeared in Bergmann glial fibers (right-pointing arrows) and outlines of molecular layer neurons (left-pointing arrows). B. Pre-incubation of the antibody with Kir2.2/KCNJ12 Blocking Peptide (BLP-PC042), suppressed staining. Cell nuclei are stained with DAPI (blue). MOL = molecular layer.
Western blot analysis of rat brain (lanes 1 and 4) mouse brain (lanes 2 and 5) and rat stomach (lanes 3 and 6) lysates: - 1-3. Anti-xCT/SLC7A11 (extracellular) Antibody(#ANT-111) (1:800).4-6. Anti-xCT/SLC7A11 (extracellular) Antibody preincubated with the negative control antigen.
quantity:
price:
to the supplier - domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry - paraffin section - mouse monoclonal (S124B-38)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
gene information - human KCNJ12
- synonym:IRK-2; IRK2; KCNJN1; Kir2.2; Kir2.2v; hIRK; hIRK1; hkir2.2x; kcnj12x
- description:potassium inwardly rectifying channel subfamily J member 12
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- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
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