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- domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunocytochemistry, enzyme immunoassay, chromatin immunoprecipitation
Western Blot. Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate. All lanes: HIST1H1B antibody at 1.5ug/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 23 KDa. Observed band size: 32 KDa.
Chromatin Immunoprecipitation Hela (4*10 6 , treated with 100nM calyculin A for 60min) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5ug anti-HIST1H1B (MBS7106712) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the Beta-Globin promoter.
Immunofluorescence staining of HepG2 cells with MBS7106712 at 1:100, counter?stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488?congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
quantity: 0.05 mL
price:
to the supplier - domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, enzyme immunoassay 
Western Blot. Positive WB detected in: Rat brain tissue, Rat kidney tissue, Mouse brain tissue, Mouse spleen tissue. All lanes: HIST1H1B antibody at 1.5ug/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 23 kDa. Observed band size: 23 kDa.
Immunohistochemistry of paraffin-embedded human colon cancer using MBS7106718 at dilution of 1:100
Immunohistochemistry of paraffin-embedded human melanoma cancer using MBS7106718 at dilution of 1:100
quantity: 0.05 mL
price:
to the supplier - domestic rabbit polyclonal
reactivity: human
application: western blot
gene information - human H1-5
- synonym:H1; H1.5; H1B; H1F5; H1s-3; HIST1H1B
- description:H1.5 linker histone, cluster member
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- we manually curate antibody information from the literature to obtain a comprehensive set of well-validated antibodies.
- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
Article
product type
- H1-5 antibody
- H1-5 gene knockdown
- H1-5 cDNA
- H1-5 other
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