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- domestic rabbit polyclonal
reactivity: human
application: western blot - domestic rabbit polyclonal
reactivity: human
application: western blot, flow cytometry
Western blot analysis of PRDC on CAL62 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500225, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Flow cytometric analysis of PRDC was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (HA500225, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control (green). After incubation of the primary antibody at +4℃ for 1 hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃ (dark incubation).Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 330 USD
to the supplier - domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry - paraffin section
gene information - human GREM2
- synonym:CKTSF1B2; DAND3; PRDC; STHAG9
- description:gremlin 2, DAN family BMP antagonist
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