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- domestic rabbit monoclonal (PSH07-77)
reactivity: human
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of Pet1 on different lysates with Rabbit anti-Pet1 antibody (HA722893) at 1/2,000 dilution. Lane 1: 293T transfected with empty control cell lysate Lane 2: 293T transfected with Pet1 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 25 kDa Observed band size: 30 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722893) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of HeLa transfected with Pet1 cells labeling Pet1 with Rabbit anti-Pet1 antibody (HA722893) at 1/50,000 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Pet1 antibody (HA722893) at 1/50,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded HeLa transfected with Pet1 cells with Rabbit anti-Pet1 antibody (HA722893) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722893) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier - domestic rabbit monoclonal (PSH07-77)
reactivity: human
application: western blot, immunohistochemistry - paraffin section 
Western blot analysis of Pet1 on different lysates with Rabbit anti-Pet1 antibody (HA751189) at 1/2,000 dilution. Lane 1: 293T transfected with empty control cell lysate Lane 2: 293T transfected with Pet1 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 25 kDa Observed band size: 30 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751189) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of HeLa transfected with Pet1 cells labeling Pet1 with Rabbit anti-Pet1 antibody (HA751189) at 1/50,000 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Pet1 antibody (HA751189) at 1/50,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded HeLa transfected with Pet1 cells with Rabbit anti-Pet1 antibody (HA751189) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751189) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 649.00 USD
to the supplier - domestic rabbit polyclonal
reactivity: human
application: western blot - domestic rabbit monoclonal
reactivity: human
application: western blot - domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, enzyme immunoassay, immunohistochemistry - paraffin section 
MBS9215640 staining FEV in human smallintestine sections by Immunohistochemistry(IHC-P - paraformaldehyde-fixed,paraffin-embedded sections). Tissue was fixedwith formaldehyde and blocked with 3% BSAfor 0. 5 hour at room temperature; antigenretrieval was by heat mediation with a citratebuffer (pH6). Samples were incubated withprimary antibody (1/25) for 1 hours at 37°C. Aundiluted biotinylated goat polyvalent antibodywas used as the secondary antibody.
Anti-FEV Antibody (N-Term) at 1:2000 dilution+ K562 whole cell lysate Lysates/proteins at 20?g per lane. Secondary Goat Anti-Rabbit IgG,(H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 25 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
quantity: 0.05 mL
price:
to the supplier
gene information - human FEV
- synonym:HSRNAFEV; PET-1
- description:FEV transcription factor, ETS family member
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- FEV antibody
- FEV gene knockdown
- FEV cDNA
- FEV protein
- FEV other
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