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- domestic rabbit polyclonal
reactivity: human
application: immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
citations: 1

Immunocytochemistry/ Immunofluorescence: CTU2 Antibody [NBP1-88457]
quantity: 0.1 ml (also 25 ul)
price: 499 USD
to the supplier - domestic rabbit monoclonal (PSH04-05)
reactivity: human, mouse
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of CTU2 on different lysates with Rabbit anti-CTU2 antibody (HA722086) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HT-29 cell lysate Lane 3: 293T cell lysate Lane 4: K-562 cell lysate Lane 5: Raji cell lysate Lane 6: HepG2 cell lysate Lane 7: COS-1 cell lysate Lane 8: NIH/3T3 cell lysate Lane 9: Mouse spleen tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 56 kDa Observed band size: 54/56 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722086) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-CTU2 antibody (HA722086) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722086) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier - domestic rabbit monoclonal (PSH04-05)
reactivity: human, mouse
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of CTU2 on different lysates with Rabbit anti-CTU2 antibody (HA750928) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HT-29 cell lysate Lane 3: 293T cell lysate Lane 4: K-562 cell lysate Lane 5: Raji cell lysate Lane 6: HepG2 cell lysate Lane 7: COS-1 cell lysate Lane 8: NIH/3T3 cell lysate Lane 9: Mouse spleen tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 56 kDa Observed band size: 54/56 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750928) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-CTU2 antibody (HA750928) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750928) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 649.00 USD
to the supplier
gene information - human CTU2
- synonym:C16orf84; MFRG; NCS2; UPF0432
- description:cytosolic thiouridylase subunit 2
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product type
- CTU2 antibody
- CTU2 gene knockdown
- CTU2 cDNA
- CTU2 protein
- CTU2 other
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