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Western Blot using Humanized Recombinant Ipilimumab (Yervoy) Anti-human CTLA4 Fab fragment Antibody to detect extracellular domain of CTLA4 protein. MW: Molecular Weight Ladder. Lanes 1, 3, and 5: CTLA-4 reduced. Lanes 2, 4, 6: CTLA-4 non-reduced. Protein Load: Lane 1-2: 200ng. Lane 3-4: 40ng. Lane 5-6: 8ng. Primary Antibody: Anti-CTLA4 Fab fragment Antibody at 1.0µg/mL for 2 hours at 4°C. Secondary Antibody: F(ab')2 Human IgG F(ab')2 Antibody Peroxidase Conjugated Pre-Adsorbed (p/n 709-1318) at 1:5,000 dilution. The protein has a calculated MW of 18.8kDa. The protein migrates ~23-30kDa under reducing (R) conditions and ~45-60kDa in a native form in SDS-PAGE due to glycosylation.
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gene information - human CTLA 4
- synonym:ALPS5; CD; CD152; CELIAC3; CTLA-4; GRD4; GSE; IDDM12
- description:cytotoxic T-lymphocyte associated protein 4
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