Your Filters
product
- domestic goat polyclonal
reactivity: human, mouse
conjugate: biotin
application: western blot, ELISA - domestic rabbit monoclonal (PSH11-28)
reactivity: human, mouse
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of CCL3 / MIP-1 alpha on different lysates with Rabbit anti-CCL3 / MIP-1 alpha antibody (HA723310) at 1/2,000 dilution. Lane 1: THP-1 cell lysate Lane 2: THP-1 treated with 100nM TPA overnight then add 100ng/mL LPS for 7 hours then add 1μg/mL BFA for 3 hours cell lysate Lane 3: RAW264.7 cell lysate Lane 4: RAW264.7 treated with 100nM TPA overnight then add 100ng/mL LPS for 7 hours then add 1μg/mL BFA for 3 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 10 kDa Observed band size: 10 kDa Exposure time: 25 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723310) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of CCL3 / MIP-1 alpha on different lysates with Rabbit anti-CCL3 / MIP-1 alpha antibody (HA723310) at 1/2,000 dilution. Lane 1: NK-92 cell lysate (20 µg/Lane) Lane 2: HeLa cell lysate (negative) (20 µg/Lane) Predicted band size: 10 kDa Observed band size: 10 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723310) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of RAW264.7 cells untreated / treated with 100nM TPA overnight then add 100ng/mL LPS for 7 hours labeling CCL3 / MIP-1 alpha with Rabbit anti-CCL3 / MIP-1 alpha antibody (HA723310) at 1/2,000 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CCL3 / MIP-1 alpha antibody (HA723310) at 1/2,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 385.00 USD
to the supplier - domestic rabbit monoclonal (PSH11-28)
reactivity: human, mouse
application: western blot, immunohistochemistry - paraffin section 
Western blot analysis of CCL3 / MIP-1 alpha on different lysates with Rabbit anti-CCL3 / MIP-1 alpha antibody (HA751407) at 1/2,000 dilution. Lane 1: THP-1 cell lysate Lane 2: THP-1 treated with 100nM TPA overnight then add 100ng/mL LPS for 7 hours then add 1μg/mL BFA for 3 hours cell lysate Lane 3: RAW264.7 cell lysate Lane 4: RAW264.7 treated with 100nM TPA overnight then add 100ng/mL LPS for 7 hours then add 1μg/mL BFA for 3 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 10 kDa Observed band size: 10 kDa Exposure time: 25 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751407) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of CCL3 / MIP-1 alpha on different lysates with Rabbit anti-CCL3 / MIP-1 alpha antibody (HA751407) at 1/2,000 dilution. Lane 1: NK-92 cell lysate (20 µg/Lane) Lane 2: HeLa cell lysate (negative) (20 µg/Lane) Predicted band size: 10 kDa Observed band size: 10 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751407) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of RAW264.7 cells untreated / treated with 100nM TPA overnight then add 100ng/mL LPS for 7 hours labeling CCL3 / MIP-1 alpha with Rabbit anti-CCL3 / MIP-1 alpha antibody (HA751407) at 1/2,000 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CCL3 / MIP-1 alpha antibody (HA751407) at 1/2,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 649.00 USD
to the supplier - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: ELISA, flow cytometry, immunohistochemistry - paraffin section 
Immunocytochemical staining of PANC-1 cells using anti-CCL3 rabbit polyclonal antibody.
Immunocytochemical staining of A549 cells using anti-CCL3 rabbit polyclonal antibody.
Immunohistochemical analysis of paraffin- embedded mouse lung tissue using anti-CCL3 rabbit polyclonal antibody.
quantity: 100μl
price: 330 USD
to the supplier - domestic goat polyclonal
reactivity: human
application: western blot, ELISA, enzyme immunoassay
gene information - human CCL3L3
- synonym:464.2; D17S1718; G0S19-2; LD78; LD78BETA; SCYA3L; SCYA3L1
- description:C-C motif chemokine ligand 3 like 3
do you know?
- we limit the listing of re-branded antibodies to provide our visitors with accurate information and the best experience
- we manually curate antibody information from the literature to obtain a comprehensive set of well-validated antibodies.
- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
product type
- CCL3L3 antibody
- CCL3L3 gene knockdown
- CCL3L3 cDNA
- CCL3L3 other
linkout
questions and comments