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- domestic rabbit polyclonal
reactivity: human
application: western blot - domestic rabbit polyclonal
reactivity: human
application: western blot, immunoprecipitation - domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA
Western blot using Rockland's Affinity Purified anti-Elg1 antibody shows detection of a band ~120 kDa corresponding to human Elg1 (arrowhead) in various cell lysates. Lanes contain ~ 5 ?g of HeLa nuclear extract (1), HeLa (2), A431 (3), Jurkat (4) and HEK293 (5) whole cell lysates. After SDS-PAGE, transfer and blocking, the membrane was probed with the primary antibody diluted to 1:500. The membrane was then washed and reacted with a HRP conjugated Gt-a-Rabbit IgG [H&L] MX followed by ECL detection using a 2 m exposure time. The expected molecular weight of Elg1 is 120kDa according to Kanellis P et al. 2003, although the predicted molecular weight is 207 kDa. The 50kD bands in Jurkat and 293 cell lysates are probably cross-reaction with other proteins. Both the 120 kDa and 50 kDa bands are not observed when antibody is pre-incubated with peptide (data not shown).
quantity: 100 µg
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gene information - human ATAD5
- synonym:C17orf41; ELG1; FRAG1
- description:ATPase family AAA domain containing 5
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