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- domestic rabbit polyclonal
reactivity: fruit fly
modification: phosphorylated
application: western blot, ELISA, immunohistochemistry
citations: 44
Western blot using Rockland's affinity purified anti-histone H2AvD pS137 antibody shows detection of a band at ~15 kDa corresponding to phosphorylated H2AvD (lane 2 arrow-head). Lanes contain either mock-irradiated (lane 1) or 4000-RAD gamma irradiated (lane 2) Drosophila melanogaster (3rd instar) larvae brain WC lysate separated on by SDS-PAGE and transferred to nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:500. Washes and reaction with secondary antibody followed incubation. Use HRP conjugated Gt-a-Rabbit IgG [H&L] MX (611-103-122) and ECL for detection. Personal Communication. Yikang Rong, NIH, CCR, Bethesda, MD.
Immunohistochemistry with anti-Histone Antibody Tissue: Human Bladder Cancer Fixation: FFPE buffered formalin 10% conc Ag Retrieval: HIER citrate buffer pH6 or HIER EDTA pH9 Primary antibody: 2 ug/ml at 2 hr Secondary Ab: anti rabbit polymer HRP 20 ‘ RT
quantity: 100
price: 499.00 USD
to the supplier - domestic rabbit polyclonal
reactivity: fruit fly
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
citations: 1 
Western Blot: Histone H2Av [p Ser137] Antibody [NBP1-78103] - Shows detection of a band at ~15 kDa corresponding to phosphorylated H2AvD (lane 2 arrow-head). Lanes contain either mock-irradiated (lane 1) or 4000-RAD gamma irradiated (lane 2) Drosophila melanogaster (3rd instar) larvae brain WC lysate separated on by SDS-PAGE and transferred to nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:500. Washes and reaction with secondary antibody followed incubation. Use HRP conjugated Gt-a-Rabbit IgG [H&L] MX and ECL for detection. Personal Communication. YikA/G Rong, NIH, CCR, Bethesda, MD.
quantity: 0.1 mg
price: 549 USD
to the supplier - domestic rabbit polyclonal
reactivity: fruit fly
application: western blot, ELISA - mouse monoclonal (UNC93-5.2.1)
reactivity: fruit fly
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
citations: 15
gene information - fruit fly His2Av
- synonym:5499; CG5499; DmelCG5499; H2A; H2A.F/Z; H2A.V; H2A.X; H2A.Z; H2AV; H2AV_DROM; H2AX; H2AZ; H2Av; H2AvD; H2a.V; H2av; H2avD; HIS; HIS2AV; HIS2AVD; His; His2; His2A; His2AV; His2AvD; His2av; HisH2Av; Hist; Hist2av; gamma-H2AV; gamma-H2Av; gamma-H2AvD; gamma-HIS2AV; gamma-His2Av; gammaH2AV; gammaH2Av; gammaH2Ax; h2AvD; his; his2Av; l(3)05146; l(3)810; l(3)97Dd; l(3)L1602
- description:Histone H2A variant
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product type
- His2Av antibody
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