Your Filters
product
- domestic rabbit monoclonal (SA30-01)
reactivity: human, mouse, rat, chicken, zebrafish , fruit fly
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
Western blot analysis of GAPDH on Hela cell lysates with Rabbit anti-GAPDH antibody (ET1601-4). Hela cell lysates at 10 µg/Lane. Predicted band size: 36 kDa Observed band size: 36 kDa 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-4) at serial dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Western blot analysis of GAPDH on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-4, 1/100,000) was used in 5% NFDM/TBST at room temperature for 1 hour. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/200,000 dilution was used for 45 mins at room temperature. Positive control: Lane 1: Rat liver tissue lysate, 20 µg/Lane Lane 2: Rat lung tissue lysate, 20 µg/Lane Lane 3: Rat lung tissue lysate, 20 µg/Lane Lane 4: Rat heart tissue lysate, 20 µg/Lane Lane 5: Rat cerebellum tissue lysate, 20 µg/Lane Lane 6: Rat skeletal muscle tissue lysate, 20 µg/Lane Lane 7: Rat spleen tissue lysate, 20 µg/Lane Lane 8: Rat small intestine tissue lysate, 20 µg/Lane
Western blot analysis of GAPDH on different lysates with Rabbit anti-GAPDH antibody (ET1601-4) at 1/80,000 dilution. Cell lysates at 10 µg/Lane. Predicted band size: 36 kDa Observed band size: 36 kDa Exposure time: 1 minute; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-4) at 1/80,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
quantity: 50μl
price: 205.00 USD
to the supplier - domestic rabbit monoclonal (PD00-07)
reactivity: human, mouse, rat, zebrafish , fruit fly
application: western blot, flow cytometry, immunohistochemistry - paraffin section 
Western blot analysis of GAPDH on different lysates with Rabbit anti-GAPDH antibody (HA721136) at 1/20,000 dilution. Lane 1: HEK-293 cell lysate (20 µg/Lane) Lane 2: HeLa cell lysate (20 µg/Lane) Lane 3: A431 cell lysate (20 µg/Lane) Lane 4: K-562 cell lysate (20 µg/Lane) Lane 5: RAW264.7 cell lysate (20 µg/Lane) Lane 6: PC-12 cell lysate (20 µg/Lane) Lane 7: Mouse heart tissue lysate (40 µg/Lane) Lane 8: Rat heart tissue lysate (40 µg/Lane) Lane 9: Human liver tissue lysate (40 µg/Lane) Lane 10: Mouse liver tissue lysate (40 µg/Lane) Lane 11: Rat liver tissue lysate (40 µg/Lane) Lane 12: Human kidney tissue lysate (40 µg/Lane) Lane 13: Mouse kidney tissue lysate (40 µg/Lane) Lane 14: Rat kidney tissue lysate (40 µg/Lane) Predicted band size: 36 kDa Observed band size: 36 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721136) at 1/20,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of GAPDH on different lysates with Rabbit anti-GAPDH antibody (HA721136) at 1/20,000 dilution. Lane 1: E.coli cell lysate (10 µg/Lane) Lane 2: E.coli cell lysate (no heat) (10 µg/Lane) Notice: no heat means the lysate is not boiled. Predicted band size: 36 kDa Observed band size: 36 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721136) at 1/20,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of GAPDH on HepG2 cell lysates with Rabbit anti-GAPDH antibody (HA721136) at different dilutions. Lysates/proteins at 10 µg/Lane. Predicted band size: 36 kDa Observed band size: 36 kDa Exposure time: 30 seconds; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721136) at different dilutions were used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier - domestic rabbit monoclonal (SA30-01)
reactivity: human, mouse, rat, chicken, zebrafish , fruit fly
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section 
Western blot analysis of GAPDH on Hela cell lysates with Rabbit anti-GAPDH antibody (HA750022). Hela cell lysates at 10 µg/Lane. Predicted band size: 36 kDa Observed band size: 36 kDa 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750022) at serial dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Western blot analysis of GAPDH on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750022, 1/100,000) was used in 5% NFDM/TBST at room temperature for 1 hour. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/200,000 dilution was used for 45 mins at room temperature. Positive control: Lane 1: Rat liver tissue lysate, 20 µg/Lane Lane 2: Rat lung tissue lysate, 20 µg/Lane Lane 3: Rat lung tissue lysate, 20 µg/Lane Lane 4: Rat heart tissue lysate, 20 µg/Lane Lane 5: Rat cerebellum tissue lysate, 20 µg/Lane Lane 6: Rat skeletal muscle tissue lysate, 20 µg/Lane Lane 7: Rat spleen tissue lysate, 20 µg/Lane Lane 8: Rat small intestine tissue lysate, 20 µg/Lane
Western blot analysis of GAPDH on different lysates with Rabbit anti-GAPDH antibody (HA750022) at 1/80,000 dilution. Cell lysates at 10 µg/Lane. Predicted band size: 36 kDa Observed band size: 36 kDa Exposure time: 1 minute; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750022) at 1/80,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 649.00 USD
to the supplier
gene information - fruit fly Gapdh1
- synonym:BEST:GH12586; CG12055; DmelCG12055; GA3PDH; GADPH; GAP; GAPDH; GAPDH I; GAPDH-1; GAPDH1; GAPDHI; Gapd; Gapdh; Gapdh-1; Gapdh43E; gapdh; gapdh-1; gapdh1; gh12586
- description:Glyceraldehyde 3 phosphate dehydrogenase 1
do you know?
- we limit the listing of re-branded antibodies to provide our visitors with accurate information and the best experience
- we manually curate antibody information from the literature to obtain a comprehensive set of well-validated antibodies.
- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
product type
- Gapdh1 antibody
species
linkout
questions and comments