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- mouse monoclonal (CTC03 (2B5))
reactivity: human, mouse, dog, cow, equine
application: ELISA, immunocytochemistry, immunoprecipitation, flow cytometry
citations: 2
Immunofluorescent analysis of cytochrome c (green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were left untreated (left panel) or treated with 1?M staurosporine (right panel) for 3 hours and probed with a cytochrome c monoclonal antibody (Product # MA5-11823), at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with Dylight 554 phalloidin (Product # 21834), and nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan and ToxInsight at 20X magnification.
Immunoprecipitation of Cytochrome C was performed on HeLa cell lysates prepared in RIPA buffer (native conditions). Antigen-antibody complexes were formed by incubating 500ug of HeLa cell lysate in 1ml total volume with 2ug of a Cytochrome C monoclonal antibody (Product # MA5-11823), preincubated with 10ul of protein A sepharose overnight on a rocking platform at 4°C, for at least 1 hour at 4?C. The immune complexes were washed extensively and eluted with 1X SDS sample buffer. Samples were resolved on a SDS-PAGE gel, transferred to a membrane, blocked, and probed with a set of appropriate antibodies and reagents to detect Cytochrome C.
quantity: 500µL
price: 380 USD
to the supplier - mouse monoclonal (SJL2-4)
reactivity: equine
application: western blot - mouse monoclonal (7H8.2C12)
reactivity: equine
application: western blot, immunohistochemistry - paraffin section
gene information - equine CYCS
- synonym:cytochrome c; cytochrome c-like protein
- description:cytochrome c, somatic
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product type
- CYCS antibody
- CYCS cDNA
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