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- domestic goat polyclonal
reactivity: human, mouse, rat, dogs, bovine, swine, domestic sheep
application: western blot, ELISA, immunocytochemistry, immunohistochemistry - paraffin section - domestic rabbit polyclonal
reactivity: domestic sheep
application: western blot
Published customer image: . Representative immunofluorescence images of 14-3-3 isoforms in oocytes and eggs isolated from adult mice. (A, B) 14-3-3 . (C, D) 14-3-3 . (E, F) 14-3-3 . (G, H) 14-3-3 . (I, J) 14-3-3 . (K, L) 14-3-3 . (M, N) 14-3-3 . Confocal sections with regions of red fluorescence indicating the corresponding isoforms studied (see Methods). The inset in N shows the same egg labeled blue with Hoechst DNA stain (non-confocal image) and confirms that the darker areas in this region of the larger image are condensed metaphase II chromosomes. Control cells were included for each isoform experiment and were imaged using the same confocal settings. Representative control oocytes and eggs are shown in bright-field (O) and fluorescence (P). 14-3-3 accumulates, in part, in the meiotic spindle in eggs as shown by simultaneous labeling with 14-3-3 (Q) and tubulin (R) antibodies. These sequential scans are merged (14-3-3 + tubulin) in (S). The scale bars represent 10 μm for all images. From: De S, Marcinkiewicz JL, Vijayaraghavan S, Kline D. Expression of 14-3-3 protein isoforms in mouse oocytes, eggs and ovarian follicular development. BMC Res Notes. 2012 Jan 23;5:57 .
quantity: 0.1 ml
price: 279 USD
to the supplier - domestic sheep polyclonal
reactivity: domestic sheep
modification: phosphorylated
application: western blot 
Western blot with Sheep anti-14-3-3 (pSER185) antibody (AHP1049). Lane 1, 2ug recombinant 14-3-3 beta. Lane 2, 2ug recombinant 14-3-3 zeta . Lane 3, sheep brain homogenate, which contains ca. 50% of beta and zeta isoforms that are phosphorylated on Ser 185. The recombinant 14-3-3s are expressed in E.coli and are not phosphorylated
quantity: 0.1 mg
price: 361 USD
to the supplier - domestic rabbit polyclonal
reactivity: domestic sheep
application: western blot, immunoprecipitation
gene information - domestic sheep SFN
- description:stratifin
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- SFN antibody
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