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- domestic rabbit polyclonal
reactivity: human, mouse, rat, dogs
application: western blot, flow cytometry, immunohistochemistry - paraffin section
Western blot analysis of Transglutaminase 2 on different lysates with Rabbit anti-Transglutaminase 2 antibody (ER1902-28) at 1/1,000 dilution. Lane 1: K-562 cell lysate Lane 2: HeLa cell lysate Lane 3: Mouse placenta tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 77 kDa Observed band size: 70-77 kDa Exposure time: 3 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1902-28) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
All lanes: Western blot analysis of Transglutaminase 2 with anti-Transglutaminase 2 antibody (ER1902-28) at 1/500 dilution. Lane 1: Wild-type MDCK whole cell lysate (10 µg). Lane 2: Transglutaminase 2 knockout MDCK whole cell lysate (10 µg). ER1902-28 was shown to specifically react with Transglutaminase 2 in wild-type MDCK cells. No band was observed when Transglutaminase 2 knockout sample was tested. Wild-type and Transglutaminase 2 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ER1902-28, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of HeLa cells labeling Transglutaminase 2 with Rabbit anti-Transglutaminase 2 antibody (ER1902-28) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Transglutaminase 2 antibody (ER1902-28) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 330 USD
to the supplier - mouse monoclonal
reactivity: human, mouse, rat, dogs
application: western blot, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
gene information - dogs TGM2
- description:transglutaminase 2
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product type
- TGM2 antibody
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