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- domestic rabbit polyclonal
reactivity: hamsters, human, rat, dogs
application: western blot, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - free floating section
citations: 1 - domestic rabbit polyclonal
reactivity: human, dogs
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
Western blot analysis of Human Brain Tissue Lysate using PA5-19644, RGS2 primary antibody at a dilution of 1 ug/ml. Blot treated with a secondary IR Dye680-conjugated Goat polyclonal anti-Rabbit antibody was used at a dilution of 1:10000.
Immunofluorescent staining of SH-SY5Y cells using PA5-19644, anti-RGS2 antibody. The cells were fixed with PFA (4%)for 10 minutes, permabilised TBS-T for 20 minutes and exposed to the primary antibody at a concentration of 1 ug/ml for 1 hour at room temp. A solution of BSA (1%), normal goat serum (10%) and glycine (0.3 M) was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody was a 448 fluorescence conjugated Goat anti-rabbit IgG (green) at a dilution of 1:1000. A WGA- 594 fluorescent conjugated stain was used to label plasma membranes (red) and the nuclei stain was DAPI (blue).
quantity: 100 ug
price: US 464.00
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gene information - dogs RGS2
- description:regulator of G protein signaling 2
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- we limit the listing of re-branded antibodies to provide our visitors with accurate information and the best experience
- we manually curate antibody information from the literature to obtain a comprehensive set of well-validated antibodies.
- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
product type
- RGS2 antibody
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