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- mouse monoclonal (3A8)
reactivity: human, mouse, dogs
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section
Western blot analysis of FOXA1 was performed by loading 25µg MDCK whole cell lysate onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. Membranes were then probed with a mouse monoclonal antibody recognizing phospho-MDCK (Product #MA1-091) at a dilution of 1:1000 overnight at 4°C on a rocking platform. Membranes were then washed in TBS-0.1%Tween 20 and probed with a goat anti-mouse-HRP secondary antibody (Product #) at a dilution of 1:10000 for at least one hour. Membranes were washed and chemiluminescent detection was performed using Pierce Super Signal West Pico (Product #34080).
Immunofluorescent analysis of FOXA1 (green) in MDCK cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were then blocked with 5% normal goat serum (Product #31873) for 15 minutes at room temperature. Cells were then probed with a mouse monoclonal antibody recognizing FOXA1 (Product#MA1-091), at a dilution of 1:100 for at least 1 hour at room temperature. Cells were then washed with PBS and incubated with DyLight 488 goat-anti-mouse secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye (Product# 62249). Images were taken on a Thermo Scientific ArrayScan at 10X magnification.
quantity: 100 ug
price: US 440.00
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gene information - dogs FOXA1
- description:forkhead box A1
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product type
- FOXA1 antibody
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