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- domestic rabbit polyclonal
reactivity: human, dogs
application: western blot, immunohistochemistry, immunohistochemistry - frozen section
Western blot analysis of Human Skeletal Muscle Tissue Lysate using PA5-19633, Aromatase primary antibody at a dilution of 1 ug/ml. Blot treated with a secondary IR Dye680-conjugated Goat polyclonal anti-Rabbit antibody was used at a dilution of 1:10000.
Immunofluorescent staining of HEPG2 cells using PA5-19633, anti-Aromatase gamma antibody. The cells were fixed with formaldehyde (4%) for 10 minutes, permabilised with BSA(1%), normal goat serum (10%) and glycine (0.3 M) in 0.1% T-BST for 1 hour and exposed to the primary antibody at a concentration of 5 ug/ml overnight at 4C. The secondary antibody was a 448 fluorescence conjugated Goat anti-rabbit IgG (green) at a dilution of 1:1000. A WGA- 594 fluorescent conjugated stain was used to label plasma membranes (red) and the nuclei stain was DAPI (blue).
quantity: 100 ug
price: US 474.00
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gene information - dogs CYP19A1
- synonym:CYP19
- description:cytochrome P450 family 19 subfamily A member 1
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- CYP19A1 antibody
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