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- mouse monoclonal (84H10)
reactivity: human, dog
application: ELISA, immunoprecipitation, flow cytometry
citations: 3
Published customer image: . ICAM-1 activates PKC but not PKC II in HMVECLs. A ) At 70% confluence, HMVECLs cells pretreated with 10 ng/ml TNF to induce ICAM-1 expression. At 24 hrs, the cells were suspended and immunolabeled with anti-ICAM-1 antibodies and examined by flow cytometry for ICAM-1 expression. B–D) HMVECLs were pretreated with TNF as in panel A. At 24 hrs, the endothelial cells were nonstimulated (NS) or stimulated with a confluent monolayer of anti-ICAM-1-coated beads or the control anti-PECAM-1-coated beads for 20 minutes in B and D or for the times indicated in C. The cells were lysed and the activation of PKC II and PKC was examined by western blot with B) anti-phospho-PKC IIThr641 or anti-PKC II or C–D) anti-phospho- PKC Thr638 or anti-PKC . Shown are representative blots. Shown are the mean SEM of 3 experiments. NT, nontreated. *, p 0.05 as compared to the nontreated (NT) groups. From: Abdala-Valencia H, Berdnikovs S, Cook-Mills JM (2012). Vitamin E Isoforms Differentially Regulate Intercellular Adhesion Molecule-1 Activation of PKC in Human Microvascular Endothelial Cells. PLoS ONE 7(7): e41054 .
quantity: 0.2 mg
price: 325 USD
to the supplier - mouse monoclonal (84H10)
reactivity: human, dog
conjugate: FITC
application: flow cytometry
citations: 1 
Published customer image: . ICAM-1 activates PKC but not PKC II in HMVECLs. A ) At 70% confluence, HMVECLs cells pretreated with 10 ng/ml TNF to induce ICAM-1 expression. At 24 hrs, the cells were suspended and immunolabeled with anti-ICAM-1 antibodies and examined by flow cytometry for ICAM-1 expression. B–D) HMVECLs were pretreated with TNF as in panel A. At 24 hrs, the endothelial cells were nonstimulated (NS) or stimulated with a confluent monolayer of anti-ICAM-1-coated beads or the control anti-PECAM-1-coated beads for 20 minutes in B and D or for the times indicated in C. The cells were lysed and the activation of PKC II and PKC was examined by western blot with B) anti-phospho-PKC IIThr641 or anti-PKC II or C–D) anti-phospho- PKC Thr638 or anti-PKC . Shown are representative blots. Shown are the mean SEM of 3 experiments. NT, nontreated. *, p 0.05 as compared to the nontreated (NT) groups. From: Abdala-Valencia H, Berdnikovs S, Cook-Mills JM (2012). Vitamin E Isoforms Differentially Regulate Intercellular Adhesion Molecule-1 Activation of PKC in Human Microvascular Endothelial Cells. PLoS ONE 7(7): e41054 .
quantity: 100 TESTS
price: 273 USD
to the supplier - mouse monoclonal (84H10)
reactivity: human, dog
application: ELISA, immunoprecipitation, flow cytometry
citations: 1 
Published customer image: . ICAM-1 activates PKC but not PKC II in HMVECLs. A ) At 70% confluence, HMVECLs cells pretreated with 10 ng/ml TNF to induce ICAM-1 expression. At 24 hrs, the cells were suspended and immunolabeled with anti-ICAM-1 antibodies and examined by flow cytometry for ICAM-1 expression. B–D) HMVECLs were pretreated with TNF as in panel A. At 24 hrs, the endothelial cells were nonstimulated (NS) or stimulated with a confluent monolayer of anti-ICAM-1-coated beads or the control anti-PECAM-1-coated beads for 20 minutes in B and D or for the times indicated in C. The cells were lysed and the activation of PKC II and PKC was examined by western blot with B) anti-phospho-PKC IIThr641 or anti-PKC II or C–D) anti-phospho- PKC Thr638 or anti-PKC . Shown are representative blots. Shown are the mean SEM of 3 experiments. NT, nontreated. *, p 0.05 as compared to the nontreated (NT) groups. From: Abdala-Valencia H, Berdnikovs S, Cook-Mills JM (2012). Vitamin E Isoforms Differentially Regulate Intercellular Adhesion Molecule-1 Activation of PKC in Human Microvascular Endothelial Cells. PLoS ONE 7(7): e41054 .
quantity: 25 µg
price: 88 USD
to the supplier - mouse monoclonal (84H10)
reactivity: human, dog
application: ELISA, immunohistochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - frozen section
citations: 3 - rabbit polyclonal
reactivity: human, dog
application: western blot
gene information - dog intercellular adhesion molecule 1
- synonym:ICAM-1; intercellular adhesion molecule 1; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor
- description:intercellular adhesion molecule 1
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product type
- intercellular adhesion molecule 1 antibody
- intercellular adhesion molecule 1 ELISA/assay
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