dog TUBA4A antibody | 9 (1 knockout-validated) products in Validated Antibody Database; 9 cited in the literature; 15 total from 4 suppliers

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Anti-alpha Tubulin antibody [DM1A] - Loading Control
Abcam
catalog: ab7291

total number of products: 4

mouse monoclonal (DM1A)
reactivity: goldfish, human, mouse, rat, dog, chicken, cow, sheep
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, immunohistochemistry knockout validation
citations: 314

quantity: 12 ml
price:
to the supplier
alpha Tubulin antibody [TU-01]
Abcam
catalog: ab7750

total number of products: 4

mouse monoclonal (TU-01)
reactivity: human, mouse, dog, cow
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry
citations: 14

quantity: 100 ug
price:
to the supplier
alpha Tubulin antibody [DM1A] (FITC) - Microtubule Marker
Abcam
catalog: ab64503

total number of products: 4

mouse monoclonal (DM1A)
reactivity: human, mouse, rat, dog, chicken, cow
application: western blot, immunocytochemistry, immunohistochemistry - frozen section
citations: 10

quantity:
price:
to the supplier
alpha Tubulin Monoclonal Antibody (DM1A)
Invitrogen
catalog: 62204

total number of products: 7

mouse monoclonal (DM1A)
reactivity: hamsters, Leishmania infantum, African green monkey, Domestic guinea pig, human, mouse, rat, dog, chicken, cow, pig, fruit fly, sheep, common platanna
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section
citations: 119

quantity: 100µL
price: US 199
to the supplier
alpha Tubulin Monoclonal Antibody (B-5-1-2)
Invitrogen
catalog: 32-2500

total number of products: 7

mouse monoclonal (B-5-1-2)
reactivity: human, mouse, rat, dog, baker's yeast, thale cress, rabbit
application: western blot, immunocytochemistry, immunohistochemistry - paraffin section
citations: 59

quantity: 100µg
price: US 345
to the supplier
alpha Tubulin Polyclonal Antibody
Invitrogen
catalog: PA5-19489

total number of products: 7

rabbit polyclonal
reactivity: Chinese hamster, human, mouse, rat, dog, chicken, cow, fruit fly
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
citations: 4

Immunoprecipitation of acetylated alpha-Tubulin was performed using whole cell lysates from cells left untreated (DMSO only) or cells treated with 0.3uM or 3uM Trichostatin A (TSA) for 16 hours. Antigen-antibody complexes were formed by incubating 500ug of lysate with 3ug of an Acetyl Lysine monoclonal antibody (Product # MA1-2021) overnight on a rocking platform at 4°C. The immune complexes were captured on 50ul Protein A/G Agarose (Product # 20421), washed extensively, and eluted with 5X Lane Marker Reducing Sample Buffer (Product # 39000). Samples were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBS-0.1%Tween for at least 1 hour. The membrane was probed with an alpha-Tubulin polyclonal antibody (Product # PA5-19489) at a dilution of 1:1000 overnight rotating at 4°C, washed in TBST, and probed with Clean-blot IP Detection Reagent (Product # 21230) at a dilution of 1:2000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34087).

Western blot analysis of HeLa Whole Cell Lysate using PA5-19489, alpha Tubulin primary antibody at a dilution of 1 ug/ml (lane 1). PA5-19489 staining of Jurkat Whole Cell Lysate at a dilution of 1 ug/ml (Lane 2). PA5-19489 staining of A431 Whole Cell Lysate at a dilution of 1 ug/ml (lane 3). Blot treated with a secondary HRP-conjugated Goat polyclonal anti-Rabbit antibody was used at a dilution of 1:3000.

Immunofluorescent staining of HeLa cells using PA5-19489, anti-Alpha Tubulin antibody. The cells were fixed with methanol (100%) for 5 minutes and exposed to the primary antibody at a concentration of 1 ug/ml for 1 hour at room temp. The secondary antibody was a 448 fluorescence conjugated Goat anti-rabbit IgG (green) at a dilution of 1:1000. A WGA- 594 fluorescent conjugated stain was used to label plasma membranes (red) and the nuclei stain was DAPI (blue).
quantity: 50µg
price: US 390
to the supplier
Anti-TUBA4A
SICGEN
catalog: AB0046-200

goat polyclonal
reactivity: human, mouse, rat, dog, cow
application: western blot, immunocytochemistry

quantity: 400 µg
price: 300 euros
to the supplier
Anti-TUBA4A
SICGEN
catalog: AB0046-500

goat polyclonal
reactivity: human, mouse, rat, dog, cow
application: western blot, immunocytochemistry

quantity: 1 mg
price: 450 euros
to the supplier
Anti-TUBA4A
SICGEN
catalog: AB0134-200

goat polyclonal
reactivity: human, mouse, rat, dog, cow
application: western blot

quantity: 600 µg
price: 300 euros
to the supplier
alpha Tubulin Antibody
Abbexa
catalog: abx019012

mouse monoclonal
reactivity: human, mouse, rat, dog, chicken
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

quantity: 100 µg
price: 282.75 USD
to the supplier